Cancer during pregnancy alters the activity of rat placenta and enhances the expression of some apoptotic precursors
نویسندگان
چکیده
Background: The presence of cancer makes it difficult to predict the progress of pregnancy and can be deleterious to the maternal-foetal relationship. Apoptosis may affect a range of placental functions and result in the retardation of foetal growth. In this work, we investigated the placental alterations produced by tumour growth and the effects on the expression of apoptotic precursors in placental tissue. Methods: Adult female Wistar rats (90 days old, n=54) were allocated into control (C), tumour-bearing (W), or ascitic fluid-injected (A) groups and were killed on the 16, 19 or 21 day of pregnancy. Placental tissues were analysed using biochemical and histochemical assays. Results: The placental protein content and glutathione-S-transferase activity were decreased in groups W and A. Histochemical analysis showed an increase in the number of cells with cleaved PARP, caspase 3 and cytochrome c in groups W and A, indicating that the presence of tumour’s effects clearly damaged placental tissue and affected the levels of apoptotic precursors. These results were confirmed by western blotting. Conclusion: Since trophoblastic cells are responsible for maintaining a normal placental function, the uncontrolled death of these cells in response to tumour cell growth or substances derived from ascitic fluid could have a negative impact on foetal development. Further knowledge of these events may help to preserve the foetus and placenta during development.
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